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By contrast, a more recent study demonstrated that ethyl pyruvate, which has multi-effects including antibacterial, anti-inflammatory, antiviral, vasodilatory, antioxidant, and antiapoptotic effects, decreases ALT, AST, hepatic morphological changes, triglycerides, free fatty acids, and the expression of proinflammatory factors and increases the expression of anti-inflammatory factors and peroxisome proliferator-activated receptor-α mRNA which through downregulation of the ROS–Nrf2 signaling pathway, thereby alleviating ALD in mice (Fawcett et al., 1999
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Then, the CLSM images were captured using a Leica DM4000 B epifluorescence microscope