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The biosynthesis of MSH proceeds from myo-inositol-1-phosphate, UDP-GlcNAc and cysteine and occurs in five steps (Jothivasan and Hamilton, 2008)

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Then, membranes were incubated with appropriate secondary antibodies, HRP-goat-anti-mouse (Abcam, ab6789) or HRP-goat-anti-rabbit (Abcam, ab6721), to bind the primary antibodies for 1 h at room temperature

absorbed deep into the skin and helps inhibit melanin synthesis