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As proxies for liver damage, the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in serum samples using Alanine Aminotransferase Activity Assay Kit (MAK052, Sigma-Aldrich) and Aspartate Aminotransferase Activity Assay Kit (MAK055, Sigma-Aldrich), respectively, according to the manufacturer’s instructions and adding 3 μL and 5 μL of serum per well for ALT and AST activity, respectively
